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s mutans atcc 25175  (ATCC)


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    ATCC s mutans atcc 25175
    S Mutans Atcc 25175, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s+mutans+atcc+25175/Streptococcus+mutans+Clarke/pm42148817-136-14-16
    Average 99 stars, based on 2875 article reviews
    s mutans atcc 25175 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Infection:

    Article Title: The Penetration Depth and Bactericidal Efficacy of 2% Chlorhexidine Iontophoresis in Human Coronal Dentin In Vitro
    Article Snippet: .. To validate the infection method, the other 6 tooth specimens were infected with S mutans ATCC 25175 by the centrifugation method described in the previous section. ..

    Centrifugation:

    Article Title: The Penetration Depth and Bactericidal Efficacy of 2% Chlorhexidine Iontophoresis in Human Coronal Dentin In Vitro
    Article Snippet: .. To validate the infection method, the other 6 tooth specimens were infected with S mutans ATCC 25175 by the centrifugation method described in the previous section. ..

    other:

    Article Title: The in-vitro effect of bovine milk, and plant-based alternatives on oral microorganisms and biofilms.
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS Fig. 1 Plant-based milk alternatives influenced the growth of S. mutans ATCC 25175 most slightly, the lowest CFU counts were recorded in the presence of 10% oat drink (- 0.38 log10) (Fig. 2a).

    Article Title: Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA
    Article Snippet: K. aerogenes ATCC 13048, P. putida ATCC 12633, S. mutans ATCC 25175, S. epidermidis ATCC 14990 and M. smegmatis ATCC 14468 were purchased from ATCC.

    Article Title: The in-vitro effect of bovine milk, and plant-based alternatives on oral microorganisms and biofilms.
    Article Snippet: As for S. mutans ATCC 25175, only 10% of oat drink reduced the CFU counts of S. sobrinus ATCC 33478 after 6 h (-0.71 log10) (Fig. 2b).


    Activity Assay:

    Article Title: The in-vitro effect of bovine milk, and plant-based alternatives on oral microorganisms and biofilms.
    Article Snippet: .. Table 1 Composition milk (per 100 ml) according to information (Migros, Zurich, Switzerland) Table 2 Microorganisms and use in respective experiments Fig. 1 Growth curve (colony forming units (CFU)) of Streptococcus gordonii ATCC 10558 at 2 h and 6 h exposure to 2.5% and 10% of bovine milk and different plant-based milk alternatives (oat, almond, soy) Fig. 2 Growth curve (colony forming units (CFU); a, b) and pH (c, d) of S. mutans ATCC 25175 (a, c) and S. sobrinus ATCC 33478 (b, d) at 2 h and 6 h exposure to 2.5% and 10% bovine milk and different plant-based milk alternatives (oat, almond, soy) Fig. 3 Growth curve (colony forming units (CFU); A, B) and pH (C, D) of S. mutans ZIB1583 at 2 h and 6 h exposure to 2.5% and 10% bovine milk and different plant-based milk alternatives (oat, almond, soy) without (A, C) and with 5% sucrose (B, D) Fig. 4 Growth curve (colony forming units (CFU)) of P. gingivalis ATCC 33277 (a), BeTR4/9 (b), and Be-TR609/2 (c) and arginine specific amidolytic activity (d; only 24 h and 10% of test substance) at 4 h and 24 h exposure to 2.5% and 10% bovine milk and different plant-based milk alternatives (oat, almond, soy) Fig 5 Formation of “cariogenic” biofilm on surfaces coated with 2.5% and 10% of bovine milk and different plant-based milk alternatives (oat, almond, soy) Colony forming units (CFU) after 4 h (a), and 24 h (b), metabolic activity after 24 h (c), “mass” after 24 h (d), pH after 4 h (e) and pH after 24 h (f) in the newly formed biofilm */** p<0.05/0.01 vs. control Fig 6 Formation of “periodontal” biofilm on surfaces coated with 2.5% and 10% of bovine milk and different plant-based milk alternatives (oat, almond, soy) Colony forming units (CFU) after 4 h (a) and 48 h (b), metabolic activity after 48 h (c), and “mass” after 48 h (d) in the newly formed biofilm ..

    Control:

    Article Title: The in-vitro effect of bovine milk, and plant-based alternatives on oral microorganisms and biofilms.
    Article Snippet: .. Table 1 Composition milk (per 100 ml) according to information (Migros, Zurich, Switzerland) Table 2 Microorganisms and use in respective experiments Fig. 1 Growth curve (colony forming units (CFU)) of Streptococcus gordonii ATCC 10558 at 2 h and 6 h exposure to 2.5% and 10% of bovine milk and different plant-based milk alternatives (oat, almond, soy) Fig. 2 Growth curve (colony forming units (CFU); a, b) and pH (c, d) of S. mutans ATCC 25175 (a, c) and S. sobrinus ATCC 33478 (b, d) at 2 h and 6 h exposure to 2.5% and 10% bovine milk and different plant-based milk alternatives (oat, almond, soy) Fig. 3 Growth curve (colony forming units (CFU); A, B) and pH (C, D) of S. mutans ZIB1583 at 2 h and 6 h exposure to 2.5% and 10% bovine milk and different plant-based milk alternatives (oat, almond, soy) without (A, C) and with 5% sucrose (B, D) Fig. 4 Growth curve (colony forming units (CFU)) of P. gingivalis ATCC 33277 (a), BeTR4/9 (b), and Be-TR609/2 (c) and arginine specific amidolytic activity (d; only 24 h and 10% of test substance) at 4 h and 24 h exposure to 2.5% and 10% bovine milk and different plant-based milk alternatives (oat, almond, soy) Fig 5 Formation of “cariogenic” biofilm on surfaces coated with 2.5% and 10% of bovine milk and different plant-based milk alternatives (oat, almond, soy) Colony forming units (CFU) after 4 h (a), and 24 h (b), metabolic activity after 24 h (c), “mass” after 24 h (d), pH after 4 h (e) and pH after 24 h (f) in the newly formed biofilm */** p<0.05/0.01 vs. control Fig 6 Formation of “periodontal” biofilm on surfaces coated with 2.5% and 10% of bovine milk and different plant-based milk alternatives (oat, almond, soy) Colony forming units (CFU) after 4 h (a) and 48 h (b), metabolic activity after 48 h (c), and “mass” after 48 h (d) in the newly formed biofilm ..

    Formulation:

    Article Title: Antibacterial and Anti‐Inflammatory Properties of Citrus aurantiifolia Essential Oil–Based Mouthwash: Potential and Cytotoxicity Concerns
    Article Snippet: .. Mouthwash formulation samples containing C. aurantiifolia peel essential oil at concentrations ranging from 0.1% to 2%, chlorhexidine as the positive control, and the mouthwash formulation without essential oil as the negative control were tested against S. mutans ATCC 25175, Staphylococcus aureus ATCC 6538, Enterococcus faecalis ATCC 29212, Streptococcus oralis ATCC 6249, Streptococcus sanguinis ATCC 10556, and P. gingivalis ATCC 33277. ..

    Positive Control:

    Article Title: Antibacterial and Anti‐Inflammatory Properties of Citrus aurantiifolia Essential Oil–Based Mouthwash: Potential and Cytotoxicity Concerns
    Article Snippet: .. Mouthwash formulation samples containing C. aurantiifolia peel essential oil at concentrations ranging from 0.1% to 2%, chlorhexidine as the positive control, and the mouthwash formulation without essential oil as the negative control were tested against S. mutans ATCC 25175, Staphylococcus aureus ATCC 6538, Enterococcus faecalis ATCC 29212, Streptococcus oralis ATCC 6249, Streptococcus sanguinis ATCC 10556, and P. gingivalis ATCC 33277. ..

    Negative Control:

    Article Title: Antibacterial and Anti‐Inflammatory Properties of Citrus aurantiifolia Essential Oil–Based Mouthwash: Potential and Cytotoxicity Concerns
    Article Snippet: .. Mouthwash formulation samples containing C. aurantiifolia peel essential oil at concentrations ranging from 0.1% to 2%, chlorhexidine as the positive control, and the mouthwash formulation without essential oil as the negative control were tested against S. mutans ATCC 25175, Staphylococcus aureus ATCC 6538, Enterococcus faecalis ATCC 29212, Streptococcus oralis ATCC 6249, Streptococcus sanguinis ATCC 10556, and P. gingivalis ATCC 33277. ..



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    s mutans strain  (ATCC)
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    The effect of tetrahydrocannabinol (THC) on planktonic <t>S</t> <t>mutans</t> . (A) Growth inhibition rate (%) of S mutans cells after THC treatment. THC concentrations of 2 μg/mL and above inhibited 90% of S mutans cell growth. The experiment was performed in triplicate and repeated 3 times. AMP, ampicillin. (B) The pH of the culture medium of planktonic growing S mutans in Brain Heart Infusion with increasing concentrations of THC. The experiment was repeated 3 times but no triplicates. ** P < .01, **** P < .0001.
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    Image Search Results


    The effect of tetrahydrocannabinol (THC) on planktonic S mutans . (A) Growth inhibition rate (%) of S mutans cells after THC treatment. THC concentrations of 2 μg/mL and above inhibited 90% of S mutans cell growth. The experiment was performed in triplicate and repeated 3 times. AMP, ampicillin. (B) The pH of the culture medium of planktonic growing S mutans in Brain Heart Infusion with increasing concentrations of THC. The experiment was repeated 3 times but no triplicates. ** P < .01, **** P < .0001.

    Journal: International Dental Journal

    Article Title: In Vitro Antimicrobial Effect of Tetrahydrocannabinol on Streptococcus mutans and Its Anticariogenic Potential

    doi: 10.1016/j.identj.2025.109386

    Figure Lengend Snippet: The effect of tetrahydrocannabinol (THC) on planktonic S mutans . (A) Growth inhibition rate (%) of S mutans cells after THC treatment. THC concentrations of 2 μg/mL and above inhibited 90% of S mutans cell growth. The experiment was performed in triplicate and repeated 3 times. AMP, ampicillin. (B) The pH of the culture medium of planktonic growing S mutans in Brain Heart Infusion with increasing concentrations of THC. The experiment was repeated 3 times but no triplicates. ** P < .01, **** P < .0001.

    Article Snippet: The S mutans strain (ATCC 25175) was purchased from the American Type Culture Collection.

    Techniques: Inhibition

    The effect of tetrahydrocannabinol on S mutans biofilm formation. (A, B) The crystal violet staining was used to detect the biomass of the S mutans biofilm. (C) The live/dead assay was used to determine the viability of the S mutans biofilm. Relative fluorescent units indicate the value of each treatment group normalized to the solvent control. Those results were determined by the TECAN Spark. Each experiment was performed in triplicate and repeated 3 times. **** P < .0001.

    Journal: International Dental Journal

    Article Title: In Vitro Antimicrobial Effect of Tetrahydrocannabinol on Streptococcus mutans and Its Anticariogenic Potential

    doi: 10.1016/j.identj.2025.109386

    Figure Lengend Snippet: The effect of tetrahydrocannabinol on S mutans biofilm formation. (A, B) The crystal violet staining was used to detect the biomass of the S mutans biofilm. (C) The live/dead assay was used to determine the viability of the S mutans biofilm. Relative fluorescent units indicate the value of each treatment group normalized to the solvent control. Those results were determined by the TECAN Spark. Each experiment was performed in triplicate and repeated 3 times. **** P < .0001.

    Article Snippet: The S mutans strain (ATCC 25175) was purchased from the American Type Culture Collection.

    Techniques: Staining, Live Dead Assay, Solvent, Control

    The effect of tetrahydrocannabinol (THC) inhibited S mutans biofilm formation, and viability was examined by immunofluorescence. Each experiment was performed twice, and representative images were used. Pie charts show the proportions of integrated fluorescence intensity (IntDen, analyzed with ImageJ) corresponding to PI (red), SYTO 9 (green), and Cascade Blue Dextran (blue) signals. Scale bars represent 20 µm.

    Journal: International Dental Journal

    Article Title: In Vitro Antimicrobial Effect of Tetrahydrocannabinol on Streptococcus mutans and Its Anticariogenic Potential

    doi: 10.1016/j.identj.2025.109386

    Figure Lengend Snippet: The effect of tetrahydrocannabinol (THC) inhibited S mutans biofilm formation, and viability was examined by immunofluorescence. Each experiment was performed twice, and representative images were used. Pie charts show the proportions of integrated fluorescence intensity (IntDen, analyzed with ImageJ) corresponding to PI (red), SYTO 9 (green), and Cascade Blue Dextran (blue) signals. Scale bars represent 20 µm.

    Article Snippet: The S mutans strain (ATCC 25175) was purchased from the American Type Culture Collection.

    Techniques: Immunofluorescence, Fluorescence

    The effect of tetrahydrocannabinol (THC) on the preformed S mutans biofilm. (A, B) The crystal violet staining was used to detect the biomass of the S mutans biofilm. (C-F) The live/dead assay was used to determine the viability of the S mutans biofilm after 1, 3, 6, and 24 hours of treatment. (G) The methylthiazolyldiphenyl tetrazolium bromide (MTT) assay was used to detect the metabolic activity of the S mutans biofilm. (H, I) The timeline of the live cells and dead cells after THC treatment for 1, 3, 6, and 24 hours. Those results were determined by the TECAN Spark. Each experiment was performed 3 times in triplicate. * P < .05, ** P < .01, *** P < .001, **** P < .0001.

    Journal: International Dental Journal

    Article Title: In Vitro Antimicrobial Effect of Tetrahydrocannabinol on Streptococcus mutans and Its Anticariogenic Potential

    doi: 10.1016/j.identj.2025.109386

    Figure Lengend Snippet: The effect of tetrahydrocannabinol (THC) on the preformed S mutans biofilm. (A, B) The crystal violet staining was used to detect the biomass of the S mutans biofilm. (C-F) The live/dead assay was used to determine the viability of the S mutans biofilm after 1, 3, 6, and 24 hours of treatment. (G) The methylthiazolyldiphenyl tetrazolium bromide (MTT) assay was used to detect the metabolic activity of the S mutans biofilm. (H, I) The timeline of the live cells and dead cells after THC treatment for 1, 3, 6, and 24 hours. Those results were determined by the TECAN Spark. Each experiment was performed 3 times in triplicate. * P < .05, ** P < .01, *** P < .001, **** P < .0001.

    Article Snippet: The S mutans strain (ATCC 25175) was purchased from the American Type Culture Collection.

    Techniques: Staining, Live Dead Assay, MTT Assay, Activity Assay

    The effect of tetrahydrocannabinol (THC) on the preformed S mutans biofilm viability. Biofilms were photographed under a fluorescence microscope after live/dead staining. The live cells are shown in green, and the dead cells in red. The figure shows a merged color of green and red, evaluating the viability of S mutans biofilm. At the 1-hour, 3-hour, and 6-hour time points, compared with the methanol group, the biofilm was more yellow and even red in the THC groups, and as the THC concentration increased, the color of the biofilm became redder, indicating a dose-dependent change. In the different concentration groups, as time increased, the color of the biofilm became increasingly red from 1 to 6 hours, while there was no obvious change in the methanol group, illustrating a time-dependent change. In the 24-hour group, the color of the biofilms in the THC groups was not as red as at 6 hours, consistent with the TECAN results. As shown in Figure 4H-I, the live cells increased, and the dead cells did not change much, suggesting that the effect of THC gradually diminished over 6 to 24 hours. Each experiment was performed twice, and representative images were used. Pie charts show the proportions of integrated fluorescence intensity (IntDen, analyzed with ImageJ) corresponding to PI (red) and SYTO 9 (green) signals. Scale bars represent 20 µm.

    Journal: International Dental Journal

    Article Title: In Vitro Antimicrobial Effect of Tetrahydrocannabinol on Streptococcus mutans and Its Anticariogenic Potential

    doi: 10.1016/j.identj.2025.109386

    Figure Lengend Snippet: The effect of tetrahydrocannabinol (THC) on the preformed S mutans biofilm viability. Biofilms were photographed under a fluorescence microscope after live/dead staining. The live cells are shown in green, and the dead cells in red. The figure shows a merged color of green and red, evaluating the viability of S mutans biofilm. At the 1-hour, 3-hour, and 6-hour time points, compared with the methanol group, the biofilm was more yellow and even red in the THC groups, and as the THC concentration increased, the color of the biofilm became redder, indicating a dose-dependent change. In the different concentration groups, as time increased, the color of the biofilm became increasingly red from 1 to 6 hours, while there was no obvious change in the methanol group, illustrating a time-dependent change. In the 24-hour group, the color of the biofilms in the THC groups was not as red as at 6 hours, consistent with the TECAN results. As shown in Figure 4H-I, the live cells increased, and the dead cells did not change much, suggesting that the effect of THC gradually diminished over 6 to 24 hours. Each experiment was performed twice, and representative images were used. Pie charts show the proportions of integrated fluorescence intensity (IntDen, analyzed with ImageJ) corresponding to PI (red) and SYTO 9 (green) signals. Scale bars represent 20 µm.

    Article Snippet: The S mutans strain (ATCC 25175) was purchased from the American Type Culture Collection.

    Techniques: Fluorescence, Microscopy, Staining, Concentration Assay

    The effect of tetrahydrocannabinol (THC) on S mutans membrane potential. (A) Performance and validation of negative (black bars) and positive (gray bars) controls for the membrane potential assays performed simultaneously in a 96-well microplate under harmonized conditions (Brain Heart Infusion medium, cellular density OD 600 = 0.03) using S mutans in the absence of cannabinoids. (B) The effect of different concentrations of THC on S mutans after a 5-minute treatment. ** P < .01, **** P < .0001.

    Journal: International Dental Journal

    Article Title: In Vitro Antimicrobial Effect of Tetrahydrocannabinol on Streptococcus mutans and Its Anticariogenic Potential

    doi: 10.1016/j.identj.2025.109386

    Figure Lengend Snippet: The effect of tetrahydrocannabinol (THC) on S mutans membrane potential. (A) Performance and validation of negative (black bars) and positive (gray bars) controls for the membrane potential assays performed simultaneously in a 96-well microplate under harmonized conditions (Brain Heart Infusion medium, cellular density OD 600 = 0.03) using S mutans in the absence of cannabinoids. (B) The effect of different concentrations of THC on S mutans after a 5-minute treatment. ** P < .01, **** P < .0001.

    Article Snippet: The S mutans strain (ATCC 25175) was purchased from the American Type Culture Collection.

    Techniques: Membrane, Biomarker Discovery